purification and evaluation of glycerol teichoic acid from biofilm forming staphylococcus epidermidis as a putative vaccine candidate

Authors

b mirzaei department of microbiology, microbial research center, pasteur institute of iran, tehran, iran, microbiology research center (mrc) pasteur institute of iran, tehran, iran.

sf moosavi department of microbiology, microbial research center, pasteur institute of iran, tehran, iran, microbiology research center (mrc) pasteur institute of iran, tehran, iran.

r babaei microbiology research center (mrc) pasteur institute of iran, tehran, iran, department of mycobacteriology and pulmonary research, pasteur institute of iran, tehran, iran.

sd siadat microbiology research center (mrc) pasteur institute of iran, tehran, iran, department of mycobacteriology and pulmonary research, pasteur institute of iran, tehran, iran.

abstract

introduction: biofilm forming staphylococcus epidermidis is a main causative agent of infections related to medical devices. purification and evaluation of gly-ta polysaccharide from a biofilm-forming s. epidermidis as a putative vaccine candidate were the main goals of the current study. methods: taking advantage of size exclusion chromatography procedure, glycerol teichoic acid (gly-ta) was purified from the above-mentioned strain and biochemical analyses including, fourier transform infrared spectroscopy (ftir) and proton nuclear magnetic resonance spectroscopy (h1-nmr) were conducted for the recovered polysaccharide. results: following pcr confirmation of a s. epidermidis strain, gly-ta was extracted and its biochemical compositions (i.e. n-acetyl glucose amine residues) were obtained. conclusion: it is envisaged that gly-ta polysaccharide could be considered as a putative vaccine to inhibit formation of biofilm by s. epidermidis.

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Journal title:
vaccine research

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