purification and evaluation of glycerol teichoic acid from biofilm forming staphylococcus epidermidis as a putative vaccine candidate
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abstract
introduction: biofilm forming staphylococcus epidermidis is a main causative agent of infections related to medical devices. purification and evaluation of gly-ta polysaccharide from a biofilm-forming s. epidermidis as a putative vaccine candidate were the main goals of the current study. methods: taking advantage of size exclusion chromatography procedure, glycerol teichoic acid (gly-ta) was purified from the above-mentioned strain and biochemical analyses including, fourier transform infrared spectroscopy (ftir) and proton nuclear magnetic resonance spectroscopy (h1-nmr) were conducted for the recovered polysaccharide. results: following pcr confirmation of a s. epidermidis strain, gly-ta was extracted and its biochemical compositions (i.e. n-acetyl glucose amine residues) were obtained. conclusion: it is envisaged that gly-ta polysaccharide could be considered as a putative vaccine to inhibit formation of biofilm by s. epidermidis.
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Purification and evaluation of glycerol teichoic acid from biofilm forming Staphylococcus epidermidis as a putative vaccine candidate
Introduction: Biofilm forming Staphylococcus epidermidis is a main causative agent of infections related to medical devices. Purification and evaluation of Gly-TA polysaccharide from a biofilm-forming S. epidermidis as a putative vaccine candidate were the main goals of the current study. Methods: Taking advantage of size exclusion chromatography procedure, glycerol teichoic acid (Gly-TA) was p...
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1. Walls of Staphylococcus epidermidis I2 contain 30% (w/w) of a glycerol teichoic acid containing phosphate, d-alanine and d-glucose in the molecular proportions 1:0.25:0.50. 2. The teichoic acid was isolated by extraction with trichloroacetic acid and with dilute aqueous NN-dimethylhydrazine at pH7, and was shown to be a (1-->3)-linked poly(glycerol phosphate) containing beta-d-glucopyranosyl...
full textThe glycerol teichoic acid of walls of Staphylococcus lactis I3.
1. The teichoic acid from walls of Staphylococcus lactis I3 was isolated by extraction with trichloroacetic acid and shown to contain glycerol, N-acetylglucosamine, phosphate and d-alanine in the molecular proportions 1:1:2:1. The alanine is attached to the polymer through ester linkages. 2. Hydrolysis with acid gave alanine, glucosamine and glycerol diphosphates. Under mild acid conditions a r...
full textComparison of cell-wall teichoic acid with high-molecular-weight extracellular slime material from Staphylococcus epidermidis.
Extracellular high-mol.-wt material was separated from liquid cultures of Staphylococcus epidermidis. This material contained protein c. 20% w/w and polysaccharide c. 80% w/w. The polysaccharide was isolated by gel and ion-exchange chromatography and contained glycerol phosphate, glucose, N-acetylglucosamine, and D-alanine. Cell-wall teichoic acid was isolated from strain RP-62A and had a simil...
full textEradication of biofilm-forming Staphylococcus epidermidis (RP62A) by a combination of sodium salicylate and vancomycin.
Staphylococcus epidermidis is a major cause of infections associated with indwelling medical devices. Biofilm production is an important virulence attribute in the pathogenesis of device-related infections. Therefore, elimination of these biofilms is an ideal treatment. Salicylate (5 mM) combined with 1 microg of vancomycin per ml inhibited biofilm formation by S. epidermidis (RP62A) by >or=99....
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Journal title:
vaccine researchجلد ۲، شماره ۵، صفحات ۱۱۳-۱۱۷
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